课程文献中心 更多>>

关键词： 雨课堂   混合式教学   生物化学   糖代谢  

摘要： 生物化学作为一门学科基础课程在酿酒、葡萄与葡萄酒、食品工程、资源环境等专业都有着举足轻重的作用。但因为课程内容庞杂、理论知识点繁多、概念抽象难于记忆理解,学生在学习中缺乏兴趣和积极性,给教学工作带来了困难。顺应形势发展,本文以糖代谢为例,探讨了运用雨课堂平台,在生物化学课前、课上、课后等环节展开线上线下混合式教学改革,以激发学生的学习兴趣,促进师生互动,提高教学效果。

关键词： drought resistance   lipids   photosynthetic pigments   productivity   proline   proteins   Solanum tuberosum  

摘要： Abstract: Interrelation between the morphology, physiology, biochemistry, and productivity of potato plants was shown for the first time using the example of a mid-season-ripening variety (v.) Siversky and a mid-early Tretyakovka v. The yield of Siversky v. turned out to be 1.6 times higher than the yield of Tretyakovka. Aboveground biomass of Siversky v. was distinguished by an increased content of photosynthetic pigments, a greater variability of the protein and lipid metabolism indicators, and more intense oxidation processes and antioxidant protection, which can be the key to its greater productivity. Multivariate statistical analysis showed that the greatest relationship in the climatic conditions of central Russia in 2020 was found for productivity and such indicators as the stomata number per unit leaf area, the number of stems, and the content of pigments, phospholipids, neutral lipids, and water-soluble part of the protein. Thus, both morphological and physiological–biochemical properties can influence the course and direction of the production process, and, hence, the yield of a certain variety. © 2021, Pleiades Publishing, Ltd.

关键词： MBBS学生   教学经验   生物化学与分子生物学   昆明医科大学  

摘要： 随着"一带一路"对推进全球化发展进程的影响,作为西南地区最具特色的医科大学之一,我校在东南亚区域越来越发挥出重要的教育辐射和支撑作用,我校优良的学习环境吸引了来自印度、尼珀、缅甸、泰国、孟加拉国等南亚和东南亚国家的留学生来攻读医学和外科学士(MBBS)学位。从2013年开始,我很荣幸成为他们的生化老师,在这有趣的7年教学过程中,我对生物化学和分子生物学课程的教学有了很多的理解,积累了一些有价值的教学经验。在这篇论文中,我很高兴把这些经验分享给和我一样的同事或一些年轻的老师。

关键词： 《生物化学与生物物理进展》  

摘要： 《生物化学与生物物理进展》拟于2022年1月出版主题为"蛋白质与多肽的检测和治疗(Protein and Peptide Diagnostics and Therapeutics)"的基础研究专刊。现诚邀海内外科研工作者来稿,稿件形式为研究报告或者综述,稿件文字中英文皆可。

关键词： Agro-ecological zone   Antioxidant enzyme   Basmati or non-basmati   Rice  

摘要： India has a diverse range of agro-ecological conditions which support the cultivation of different rice varieties differing in the adaptation which is so important for sustainable development of rice crop. Specific ecotypes of rice adapted to diverse conditions have divergence in their morphology, physiology, biochemistry, molecular function, agronomy, and stress response. In the present study, 12 different rice varieties viz., PB-1, PB-1509, Pusa-RH-10, CSR-30, HKR-47, PR-126, Govind, Sharbati, ADT-37, ADT-39, ADT-45, White Ponni, were selected for the study of intrinsic biochemical behaviour and these varieties belong to different Agro-ecological zones and basmati or non-basmati rice varieties. Amongst intrinsic biochemicals activity, the differential response of radical scavenging, superoxide dismutase (SOD), catalase (CAT) and guaiacol peroxidase (POX) activities, were observed in the selected rice varieties at 14 days old seedling stage, developed under controlled growth conditions. Comparatively, North India region rice varieties displayed an enhanced intrinsic biochemical response than south India region rice varieties. Similarly, basmati rice varieties showed increased biochemical response compared to non-basmati rice varieties. Thus, the differential biochemical responses (radical scavenging, SOD, CAT, and POX activities) observed creates a significant difference between rice varieties and provides valuable information about rice ecotype-biochemical interaction for sustainable adaptive value under different ecological conditions. © 2020, Korean Society of Crop Science (KSCS).

关键词： Algae extracts   Amendment   Biostimulants   Germination   Growth  

摘要： This current work, examines the effects of three brown algae (Cystoseira gibraltarica, Fucus spiralis and Bifurcaria bifurcata) as biostimulant on the germination and the growth of tomato (Solanum lycopersicum) in vitro, in addition to the greenhouse. The different treatments (Amendment/aqueous extracts) had been tested with varied concentrations. The obtained results proved that the seeds which received the extracts of C. gibraltarica and B. bifurcata at feeble concentration (0.5%) were lightly germinated with high length of hypocotyls and radicle. The F. spiralis alga extract ameliorated the rate of germination, the length of the hypocotyls and of the radicle for the three concentrations. Moreover, all concentrations improved seedling biomass. The growth pourcentage in the greenhouse (the aerial part length, the root's length, total length and dry weights) was higher in the plants that received a weak concentrations of extracts of the algae B. bifurcata, C. gibraltarica and F. spiralis. Furthermore, the biochemical composition of plants treated with B. bifurcata and F. spiralis extract at low concentration were improved. This study came up with interesting information about F. spiralis and B. bifurcata species could be regarded as an excellent biostimulant to improve the tomato growth. © 2021 The Authors

关键词： 生物化学   习题   微信小程序  

摘要： 基于微信小程序的及时便捷和轻量化的特点,设计生物化学习题趣味小程序,该程序的开发设计了学生和教师客户端,并设置了视频教学、线上答题、论坛讨论等多个功能模块,实现了生物化学练习与学习的闭环,为学生和教师用户提供了交流、联系和资料查询平台.

关键词： Infertility   Kigelia africana   Metoclopramide   Prolactin  

摘要： To study the biochemical and hormonal parameters in metoclopramide-induced hyperprolactinemic female Wistar rats administered ethanol leaf extract of Kigelia africana. Thirty (30) female Wistar rats were grouped into two major parts: (i) normal control (n = 5) and (ii) experimental (n = 25) to induce hyperprolactinemia. The experimental group was divided into five groups of five animals each, given a total of six in all. Group 1 received the distilled water alone, group 2 was metoclopramide-induced hyperprolactinemic rats only, group 3 comprised metoclopramide-induced hyperprolactinemic rats administered bromocriptine (2.5 mg Kg–1 ***.), and groups 4–6 were metoclopramide-induced hyperprolactinemic rats administered 100, 200, and 400 mg Kg–1K. africana ethanol extract, respectively. After treatments, biochemical and hormonal parameters were determined. Prolactin level increased significantly (P < 0.05) in the experimental groups and reduced following administration of various concentrations of K. africana extract (100, 200, and 400 mg Kg–1 ***.) and bromocriptine than the normal control and hyperprolactinemic control groups. Significant increase (P < 0.05) in progesterone, luteinizing hormone follicle-stimulating hormone, and estradiol serum levels were observed at 400 mg Kg–1 ***. of the extract as against the normal control. Similarly, bromocriptine (2.5 mg Kg–1 ***.) produced the same effect. Non-significant increase (P > 0.05) of cholesterol and protein uteri levels were obtained in experimental rats treated with K. africana extract in comparison with the normal control and hyperprolactinemia control. In conclusion, ethanol extract of the plant can ameliorate hyperprolactinemia in rats and could improve some fertility hormones through its stimulatory effect on the gonads. © 2021, The Author(s), under exclusive licence to Springer-Verlag London Ltd. part of Springer Nature.

摘要： 1 《中国生物化学与分子生物学报》简介《中国生物化学与分子生物学报》(Chinese Journal of Biochemistry and Molecular Biology,CJBMB)是由中国科学技术协会主管、中国生物化学与分子生物学会和北京大学共同主办,国内外公开发行的国家生物学类/基础医学类核心期刊(月刊)。本刊被美国《化学文摘》(CA)、美国《生物学文摘》(BA)、俄罗斯《文摘杂志》(AJ)、世界卫生组织西太平洋地区医学索引(WPRIM)、中国科技论文与引文数据库(CSTPCD)、Scopus数据库、中国科学引文数据库(CSCD)、《中国科技论文统计源期刊)》、《中文核心期刊要目总览(第1～8版)》、《中国生物学文摘数据库》、《中国期刊网》、《中国学术期刊(光盘版)全文数据库》和《中国科技期刊精品数据库》等检索数据库收录。

关键词： 全无机钙钛矿纳米材料   长余辉纳米材料   荧光   SERS   食品安全  

摘要： 食品检测作为保障食品安全的关键环节,对促进经济发展和保护人类生命健康具有重要意义。不同食品基质需要特定的检测探针,油相食品基质需要前处理简单、检测结果稳定且能够适合油相检测的探针,水相食品基质需要适合水相体系下高信号强度、抗干扰和准确灵敏的检测探针。全无机钙钛矿量子点在油相基质中荧光性质稳定。长余辉纳米材料由于余辉性质可避免水相基质干扰。因此,本论文以半导体纳米材料——全无机钙钛矿量子点和长余辉纳米材料为研究对象,设计荧光探针、SERS传感器及荧光-SERS双模态传感器,实现食用油理化指标和有害因子以及水相中食源性致病菌肠毒素(Staphylococcal enterotoxins,SEC)的灵敏准确检测。本论文的主要研究工作如下:1、基于全无机钙钛矿纳米材料,构建食用油品质多模态传感分析方法。通过调控卤化铅投料比及反应时间制备了一系列不同发射的油溶性全无机钙钛矿量子点(All-inorganic perovskite quantum dots,Cs Pb X QDs)。Cs Pb BrI QDs的橙色荧光在食用油中具有良好的稳定性。基于Cs Pb BrI QDs遇酸破坏钙钛矿结构导致荧光猝灭的原理,设计荧光猝灭型传感器用于食用油的酸值检测,酸值的检出限为0.71 mg KOH/g。且基于在甲苯试剂中3-氯-1,2-丙二醇(3-MCPD)易与Cs Pb BrI QDs发生卤素交换反应的原理,构建荧光偏移型传感器用于检测食用油中的3-MCPD,检出限为39.81μg/m L。此外,基于遇水荧光易猝灭的介孔二氧化硅包覆全无机钙钛矿量子点(Cs Pb BrI@MSNs)与水稳定性的二维钙钛矿纳米片Cs Pb Br NSs,构建了检测食用油中水分含量的比率荧光探针,检出限为0.45%。制备的一系列不同性质的钙钛矿材料,实现了食用油中有害因子的便捷、灵敏和裸眼检测,为多模态油相食品安全监测提供了新材料和新途径。2、构建基于全无机钙钛矿材料的SERS传感器,用于检测水相食品基质中的SEC。通过高温去溶剂法制备绿色发射的Cs Pb Br@MSNs,介孔二氧化硅的引入使得该材料可以在水中均匀分散并为生物分子的修饰提供反应位点,且遇水转变为无荧光的Cs PbBr@MSNs半导体纳米材料。基于Cs PbBr@MSNs与Au-Ag Janus NPs复合材料构建的SERS传感器表现出增强的SERS信号。该SERS传感器可以实现牛奶样品中SEC的灵敏检测,检出限可达0.83 pg/m L。3、开发了基于长余辉纳米材料和等离子体纳米材料复合结构的荧光-SERS双模态光学传感器,用于水相基质中SEC的灵敏准确检测。通过水热法合成红色发射的长余辉纳米材料ZGGO NPs,与等离子体纳米材料Au NBPs经SEC抗体/抗原组装成复合材料SEC-composites。基于等离激元增强荧光及ZGGO NPs独特的上转换荧光增强SERS机理,SEC-composites具备增强的荧光信号及SERS信号。开发的荧光-SERS双模态光学传感器,提供了两个可以相互验证的独立检测信号,根据荧光信号和SERS信号分别得到检出限为7.5 pg/m L、8.9 pg/m L,进一步提高了SEC检测结果的准确性。该双光学信号传感器避免了食物基质的干扰并有效提高SEC检测的可靠性。