课程文献中心 更多>>

关键词： Selenium nanoparticles   kappa-carrageenan   Surfaces charge   Liver   Toxic damage   Malondialdehyde   Alanine aminotransferase   Aspartate aminotransferase   CARRAGEENAN   SIZE   AMINOTRANSFERASE   CYTOTOXICITY   BIOMARKER   DISEASE   ALANINE   CHARGE  

摘要： The ever-increasing application of selenium nanoparticles in medicine generates a need for thorough assessment of their effects on the living organism to prevent undesirable and dangerous toxic processes. The biological action of selenium nanoparticles strongly depends on the administrated dose. This stimulates in-depth study of possible mechanisms of their toxic effect, especially when they are applied in high doses. In this work, we have studied the effect of a high dose of selenium nanoparticles on rats and found that it is different in a healthy organism and in the case of modeled liver toxic damage. It has been revealed that the administration of a high dose of selenium nanoparticles to rats with toxic liver damage decreases the concentration of lipid peroxidation products in the blood and normalizes the level of liver enzymes at a time of the damage of the urinary system. At the same time, in a healthy organism, excess of selenium nanoparticles augments concentrations of urea, aspartate aminotransferase and malondialdehyde probably due to the fact that excess selenium nanoparticles induces the lipid peroxidation and damage the urinary system. However, the experiments show that, no mortality is observed among the animals treated with selenium in a dose of 6 times higher than the normal one. The use of sulfated polysaccharide kappa-carrageenan as a stabilizer of nanoparticles generates a densely negative charge (zeta-potential -29 mV) on the nanoparticles surface. Probably, the high biocompatibility of nanoparticles is explained by the prolongation of their interaction with cells due to involvement of opsonization stage of negatively charged surface of nanoparticles into their metabolism.

关键词： urinary free cortisol   late-night salivary cortisol   dexamethasone suppression test   UFC   LNSC   DST   NIGHT SALIVARY CORTISOL   URINARY FREE CORTISOL   CORTICOTROPIN-RELEASING HORMONE   AUTOMATED ELECTROCHEMILUMINESCENCE IMMUNOASSAY   DEXAMETHASONE-SUPPRESSION TEST   MIDNIGHT SERUM CORTISOL   DIFFERENTIAL-DIAGNOSIS   DESMOPRESSIN TEST   LC-MS/MS   REFERENCE INTERVALS  

摘要： Due to the variable clinical features and its rarity diagnosis of Cushing's disease (CD) is often delayed. Clearly, awareness for CD needs to be raised, accompanied by the availability of simple and accurate screening tests. Late-night salivary cortisol (LNSC), 1 mg dexamethasone suppression test (DST), and urinary free cortisol (UFC) have all been extensively studied, demonstrating high sensitivity and specificity for the diagnosis of Cushing's syndrome. However, each of those well-established tests has its own distinctive features, making it preferable in specific clinical conditions and patient groups. To choose the most appropriate test in individual patients, an expert endocrinologist should be consulted. This review will discuss the pitfalls for each of those tests. (C) 2021 Elsevier Ltd. All rights reserved.

摘要： This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://***/about/our-business/policies/article-withdrawal). This article has been retracted at the request of the Authors and the Editor-in-Chief. The paper is retracted because of a high level of duplication of "Rhodopseudomonas palustris wastewater treatment: cyhalofop-butyl removal, biochemicals production and mathematical model establishment. Bioresource. Tech. 2019, 282: 390-397. As such this article represents a severe abuse of the scientific publishing system. The scientific community takes a very strong view on this matter and apologies are offered to readers of the journal that this was not detected during the submission process. The first author, Pan Wu, takes full responsibility for these actions, a stance supported by Dalian Minzu University and Northeast Agricultural University, Harbin, where the research took place. © 2020 Elsevier Ltd

关键词： Biofilm   Extracellular polymeric substances   Corynebacterium pseudotuberculosis   Caseous lymphadenitis   Raman spectroscopy   Field emission scanning electron microscopy   CASEOUS-LYMPHADENITIS   DISINFECTANTS   BACTERIA   MATRIX   GOATS   SHEEP  

摘要： Caseous lymphadenitis (CLA) is a ruminant disease caused by Corynebacterium pseudotuberculosis, a Gram-positive facultative intracellular pathogen. The present work was performed to investigate the biochemical composition, morphology and antimicrobial susceptibility pattern of C. pseudotuberculosis biofilm using Raman spectroscopy, field emission scanning electron microscopy (FESEM) and microplate biofilm assay respectively. Results showed that the 24-h-old biofilm was characterized by Raman spectral peaks at 615 cm(-1) (CCC symmetric bend phenyl ring), 668 cm(-1) (Valine) and 825 cm(-1) (Ring breath Tyr.) whilst the 48-h-old and 72-h-old biofilms were characterized by Raman spectral peaks at 1400 cm(-1) (COO- sym.), 1450 cm(-1) (COOsym.), 1581 cm(-1) (Ring breath Trp.), 1650 cm(-1) (COO- asym.) and 1725 cm(-1) (C@O str.). Raman spectra also revealed the biochemical heterogeneity in C. pseudotuberculosis biofilm. FESEM images clearly showed the biofilm cells which were surrounded by the extracellular matrix. Treatment with nalidixic acid, streptomycin, tetracyclin, ethylenediaminetetraacetic acid (EDTA) and dimethyl sulfoxide (DMSO) significantly (p < 0.05) inhibited the viability of C. pseudotuberculosis biofilm. The present study suggests that the biochemical composition of C. pseudotuberculosis biofilm may vary across different developmental stages. Meanwhile, nalidixic acid, streptomycin, tetracyclin, EDTA and DMSO may be useful in the treatment of CLA. (C) 2020 The Author(s). Published by Elsevier B.V. on behalf of King Saud University.

关键词： Acute pancreatitis   Endoscopic ultrasound   Idiopathic pancreatitis   NONSTEROIDAL ANTIINFLAMMATORY DRUGS   RECURRENT PANCREATITIS   ENDOSCOPIC ULTRASOUND   GASTROENTERITIS   MICROLITHIASIS   MANAGEMENT   ETIOLOGY   RISK   RARE   MRCP  

摘要： Acute pancreatitis (AP) is termed as idiopathic (IAP) when the underlying conditions of pancreatic inflammation remain unknown. The aim of this study was to identify different clinical features in patients with IAP and AP of known aetiology. All patients hospitalized in our Gastroenterology Unit with an initial diagnosis of AP were recruited. AP was classified as of known aetiology or idiopathic according to clinical examination, serum biochemistry testing, and radiological imaging investigations, and clinical data in both patient groups were compared. A total of 127 patients (80 males, mean age: 57 years) were eligible for the analysis, 92 of which (73%) with AP of known aetiology and 35 (27%) with IAP. The major causes of AP were biliary obstruction (65%) or alcohol abuse (25%). Previous cholecystectomy was more frequent in patients with AP of known aetiology than in patients with IAP (14% versus 0%); patients with IAP showed lower gamma-glutamyl transpeptidase levels, lower daily alcohol intake, and higher frequency of gastroenteritis than patients with AP of known aetiology (34.3% versus 15.2%). Previous intake of nonsteroidal anti-inflammatory drugs was more frequent in patients with IAP than in patients with AP of known aetiology (23% versus 0%). No further differences in clinical features were found between the two patient groups. IAP accounts for almost 20% of cases of AP. An association of AP with gastroenteritis or the use of NSAIDs should be considered if time-related with disease onset, especially in patients with no recurrent attacks.

关键词： Red beets (Beetroots   Beta vulgaris)   Phytochemical analysis   Acetylcholinesterase (AChE) inhibitory activity   Anti-inflammatory   Antioxidant   Neurodegenerative disorders   IN-VITRO   ANTIOXIDANT PROPERTIES   ALZHEIMERS-DISEASE   OXIDATIVE STRESS   ACETYLCHOLINESTERASE   DEMENTIA   INHIBITION   DAMAGE  

摘要： Research background: Red beet (Beta vulgaris L.) is commercially grown in Punjab and Khyber Pakhtunkhwa (KPK) regions while cultivated as vegetable in Baluchistan and Sindh regions of Pakistan. It is well known for its health-promoting role in several clinical and pathologic outcome due to abundance of betalains and other bioactive compounds. The purpose of study was to interpret bioactivity of of B. vulgaris leaves (BvLM) and roots (BvRM) extracts for finding natural cure of neurodegenerative diseases (NDs). Experimental approach: BvLM and BvRM extracts were evaluated for phytochemical composition, antioxidant, anti-inflammatory and anticholinesterase potential using standard protocols with modifications. Results: Phytochemicals analysis of BvLM and BvRM extracts depicted the presence of flavonoids, phenols, alkaloids, saponins and glycosides. The IC50 values for free radical scavenging activity for BvRM and BvLM showed that BvLM (DPPH: 2.20 +/- 0.72 mu g/mL, H2O2: 0.0519 1.02 mu g/mL) is more significant as compared to BvRM (DPPH: 2.312 +/- 0.72 mu g/mL, H2O2: 2.668 +/- 0.49 mu g/mL). BvLM showed significant protection against heat induced hemolysis of HRBCs and protein denaturation (2.322 +/- 0.1 > 2.324 +/- 0. 06 mu g/mL) as compared to BvRM (8.572 +/- 0.2 > 50.18 +/- 1.0 mu g/mL). Both extracts found to exhibit strong inhibitory potential against acetylcholinesterase enzyme. Discussion/Conclusion: Our study highlighted B. vulagris extracts as rich and nutritious source of antioxidants, anti-inflammatory and cholinesterase inhibitors that could be helpful in preventing and treating neurodegenerative disorders (NDs). In consideration of multifactorial and complex etiology of NDs, BvLM and BvRM extracts would be apt candidates for development of therapeutic strategy for management of multiple cognitive disorders. (C) 2020 The Author(s). Published by Elsevier B.V. on behalf of King Saud University.

关键词： Thermococcus gammatolerans   DNA glycosylase   Deaminated DNA   Alkylated DNA   Base excision repair   3-METHYLADENINE DNA   ESCHERICHIA-COLI   SACCHAROMYCES-CEREVISIAE   OXIDATIVE DEMETHYLATION   ALKYLATION DAMAGE   STRUCTURAL BASIS   EXCISION-REPAIR   ALKA   HYPOXANTHINE   RECOGNITION  

摘要： The hyperthermophilic and radioresistant euryarchaeon Thermococcus gammatolerans encodes a putative 3-methlyadenine DNA glycosylase II (Tg-AlkA). Herein, we report biochemical characterization and catalytic mechanism of Tg-AlkA. The recombinant Tg-AlkA can excise hypoxanthine (Hx) and 1-methlyadenine (1-meA) from dsDNA with varied efficiencies at high temperature. Notably, Tg-AlkA is a bi-functional glycosylase, which is sharply distinct from all the reported AlkAs. Biochemical data show that the optimal temperature and pH of Tg-AlkA for removing Hx from dsDNA are ca.70 degrees C and ca.7.0-8.0, respectively. Furthermore, the Tg-AlkA activity is independent of a divalent metal ion, and Mg2+ stimulates the Tg-AlkA activity whereas other divalent ions inhibit the enzyme activity with varied degrees. Mutational studies show that the Tg-AlkA W204A and D223A mutants abolish completely the excision activity, thereby suggesting that residues W204 and D223 are involved in catalysis. Surprisingly, the mutations of W204, D223, Y139 and W256 to alanine in Tg-AlkA lead to the increased affinity for binding DNA substrate with varied degrees, suggesting that these residues are flexible for conformational change of the enzyme. Therefore, Tg-AlkA is a novel AlkA that can remove Hx and 1-meA from dsDNA, thus providing insights into repair of deaminated and alkylated bases in DNA from hyperthermophilic Thermococcus.

关键词： Microbial electrosynthesis   CO2 flow fluctuation   Biochemical production   CO2 valorization   Carbon capture and utilization   ACETATE   EFFICIENCY   BACTERIA  

摘要： Microbial electrosynthesis (MES) for CO2 valorization could be influenced by fluctuations in CO2 mass transfer and flow rates. In this study, we developed an efficient method for CO2 delivery to cathodic biofilm by directly sparging CO2 through the pores of ceramic hollow fiber wrapped with Ni-foam/carbon nanotube electrode, and obtained 45% and 77% higher acetate and methane production, respectively. This was followed by the MES stability test in response to fluctuations in CO2 flow rates varying from 0.3 ml/min to 10 ml/min. The biochemical production exhibited an increasing trend with CO2 flow rates, achieving higher acetate (47.0 +/- 18.4 mmol/m(2)/day) and methane (240.0 +/- 32.2 mmol/m(2)/day) generation at 10 ml/min with over 90% coulombic efficiency. The biofilm and suspended biomass, however, showed high resistance to CO2 flow fluctuations with Methanobacterium and Acetobacterium accounting for 80% of the total microbial community, which suggests the robustness of MES for onsite carbon conversion.

关键词： C. mrigala   Chlorfenapyr   Acetamiprid   Dimethoate   Histopathology and hematology   CLARIAS-GARIEPINUS BURCHELL   CYPRINUS-CARPIO   COMMON CARP   OREOCHROMIS-NILOTICUS   THYROID-HORMONES   ACUTE TOXICITY   RESPONSES   EXPOSURE   HISTOPATHOLOGY   DELTAMETHRIN  

摘要： The aim of the present study was to investigate the comparative effects of pesticides Chlorfenapyr, Dimethoate and Acetamiprid on the health of Cirrhinus mrigala under long term exposure. Eighty C. mrigala were divided in four equal groups; one control and three treated groups. The blood was collected from both control and treated groups at intervals of 10th, 20th and 30th days for hematobiochemistry and histopathological alterations. The result indicates significant difference (P < 0.05) in RBCs, Hb, PCV and MCHC whereas elevation in WBCs and Platelets counts were recorded. In 10th day sampling, MCV value of Dimethoate and Acetamiprid treatment had no difference in comparison with the control group, however it is significantly increased (P < 0.05) in rest of sampling. The MCH value of exposed fish showed significant increased (P < 0.05) after 20th and 30th days for Chlorfenapyr and after 30th days for Acetamiprid exposure while insignificantly increased for rest of sampling. It was also found that these pesticides significantly decrease (p < 0.05) the T3 and T4 levels while increase in the TSH, cortical, ALP, AST, ALT and LDH levels in the serum of the treated fishes in contrast to control group. Similarly, histopathological analysis of gills and liver showed significant alterations in all the treated groups. Toxicity trends of these pesticides was ranked as Chlorfenapyr Acetamiprid > Dimethoate. It is concluded that indiscriminate use of such pesticides poses a noxious threat to non-target organisms, harm the ecosystems and jeopardizes human health. (C) 2020 The Author(s). Published by Elsevier B.V. on behalf of King Saud University.

关键词： Ecotypic differentiation   Mesophyll conductance   Optimal temperature of photosynthesis   Temperature acclimation   Temperature adaptation   Rubisco kinetics   INDUCED DNA-DAMAGE   INTERNAL CONDUCTANCE   MESOPHYLL CONDUCTANCE   LEAF PHOTOSYNTHESIS   RUBP CARBOXYLATION   RUBISCO ACTIVASE   SEASONAL-CHANGE   SPINACH LEAVES   ACCLIMATION   C-3  

摘要： The temperature dependence of the light-saturated rate of photosynthesis has large interspecific and intraspecific variations, which may be related to adaptation to the habitat environment. As both higher latitude and altitude have lower habitat temperature, we addressed the question of whether temperature adaptation is similar between high-latitude and high-altitude ecotypes. We raised three ecotypes of Fallopia japonica collected from high latitude and low-altitude (Hirosaki), high-altitude and low-latitude (Fuji), and low-altitude and low-latitude (Tokyo) habitats at two growth temperatures, and determined the temperature dependence of photosynthesis and related biochemical and physical traits, including mesophyll conductance. Temperature dependence of photosynthesis and related traits differed among ecotypes, but the difference was not significant between Fuji and Tokyo when grown at a low-temperature. The simulation analysis suggested that the temperature dependence of Rubisco activity and the CO2 concentration in the chloroplasts had large contributions to the variation in the temperature dependence of photosynthesis. We conclude that both ecotypes from high-altitude and high latitude habitats tend to have a temperature dependence of photosynthesis which is adaptive to low-temperature conditions, but the mechanisms to adapt to high-altitude are not necessarily the same as compared with those to high-latitude.