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关键词： 焙烤食品工艺学   教学方法   教材   翻转课堂   课程思政  

摘要： “焙烤食品工艺学”课程是一门实践性很强的课程,不仅要注重基本理论知识的传授,更要注重实践操作能力的培养。在教学过程中通过自编教材和教案、优化课程内容、实施翻转课堂、建设课程思政、多样化考核方式等措施,以达到更好的教学效果、培养高水平烘焙领域技术人才。

关键词： 云班课+临床案例相结合教学模式   生物化学   应用   评价  

摘要： 本文以“物质代谢篇章”为例,构建了云班课+临床案例相结合教学模式。选取甘肃中医药大学2018级中西医临床医学专业A、B两个班级作为研究对象,A班采用云班课+临床案例教学法(实验班);B班采用传统多媒体授课法(对照班)。期末测试成绩和教学效果评价表明,实验班平均成绩优于对照班,云班课+临床案例教学法不但可以学生加深他们对课本知识的理解记忆,而且锻炼学生临床思维能力。

关键词： 冠状病毒感染   新型冠状病毒感染   儿童   肝生化异常  

摘要： 目的了解儿童及青少年新型冠状病毒(2019-nCoV)感染过程中肝功能(生化)指标变化情况。方法收集2020年1月19日至2020年11月28日复旦大学附属儿科医院收治的77例病原学确诊的2019-nCoV感染患儿的肝生化指标资料,分析其异常特征及危险因素。组间比较用t检验。结果77例2019-nCoV感染患儿中,男44例(57.1%),女33例(42.9%),中位年龄10岁。无症状感染27例(35.1%),轻型28例(36.4%),普通型22例(28.6%)。7例接受羟氯喹治疗。75例无基础疾病,其中丙氨酸转氨酶升高1例(1.5%,在使用羟氯喹过程中),天冬氨酸转氨酶升高7例(10.3%),碱性磷酸酶升高7例(28%),总胆红素、直接胆红素、白蛋白、国际标准化比值均在正常范围内。肺炎组与非肺炎组、红细胞沉降率升高组与正常组间肝生化异常差异无统计学意义(P>0.05)。1例基础疾病为胆道闭锁患儿,2019-nCoV感染后肝损伤无加重。1例基础疾病为癫痫,未出现肝生化异常。结论儿童及青少年2019-nCoV感染病例临床症状轻,肝损伤发生率低。2019-nCoV感染患儿的肝生化异常可能与其基础疾病及抗病毒治疗药物使用也相关。

关键词： 生物化学   混合式教学   教学改革与实践   教学质量  

摘要： 为了提高生物化学的教学质量,应充分利用优质的教学资源。该文认为应积极构建“MOOC+云班课+微信”线上线下混合式教学模式,辅以精品课程PPT、试题库等网络教学资源,建立多维度的教学考核评估管理体系,突出学生学习主体的地位,以实现生物化学教育教学质量新的跃升。

关键词： Endomannanase   Endoglucanase   Thermostable   alpha-Galactosidase   Synergism   BETA-MANNANASE   GENE CLONING   ENZYMATIC-HYDROLYSIS   ENDO-BETA-1,4-MANNANASE   RECOMBINANT   EXPRESSION  

摘要： A novel GH5 endo-1,4-beta-mannanase (BaMan5A) was identified from Bacillus sp. KW1, it shares the highest sequence identity (86%) with another characterized Bacillus endo-1,4-beta-mannanase. The recombinant BaMan5A displayed maximum activity at pH 7.0 and 70 degrees C, it was stable at a broad pH range (pH 3.5-11.0) after 12-h incubation at 25 degrees C, and exhibited good thermostability, retaining about 100% and 85% activity after incubating at 60 degrees C for 12 h and 65 degrees C for 8 h, respectively. The results of polysaccharide hydrolysis revealed that the enzyme can only hydrolyze mannan substrates, including carob galactomannan, konjac glucomannan, 1,4-beta-D-mannan, locust bean gum, and guar gum, yielding mannose, mannobiose, mannotriose, and some other oligosaccharides. The best substrate was carob galactomannan, the corresponding specific activity and Km value were 10,886 mu mol/min/mu mol and 3.31 mg/mL, respectively. Interestingly, BaMan5A was capable to hydrolyze both manno-oligosaccharides and cello-oligosaccharides, including mannotetraose, mannopentaose, mannohexaose, cellopentaose and cellohexaose. Furthermore, BaMan5A acted synergistically with a commercial agalactosidase (CbAgal) on galactomannan depolymerization, a best synergy degree of 1.58 was achieved after optimizing enzymeratios. This study not only expands the diversity of BacillusGH5 beta-mannanase, but also discloses the potential of BaMan5A in industrial application. (c) 2020 Elsevier B.V. All rights reserved.

关键词： Ionic liquids   phytotoxicity   Maize seedling   Antioxidant activity   Phytochemicals   THERMOPHYSICAL PROPERTIES   LIGNOCELLULOSIC BIOMASS   PETROLEUM ASPHALTENE   WASTE-WATER   METAL-IONS   TOXICITY   TETRAFLUOROBORATE   PHOTOSYSTEM   CONVERSION   CATIONS  

摘要： The structural moieties of ionic liquids (IL), as well as water miscibility nature, render it toxic in the various biological system. The ecotoxicity of animals and microbes are well established for ionic liquids. Albeit, the phytotoxicity of ILs have not been explored exclusively. Therefore, the effect of three imidazolium-based ILs, namely, 1-butyl-3-methylimidazolium chloride ([Bmim]Cl), 1-octyl-3-methylimidazolium chloride ([Omim]Cl), and 1-butyl-3-methylimidazolium tetrafluoroborate ([Bmim]BF4) on performance and biochemical constituents of maize seedling were studied. ILs in different concentrations ranging from 50 mg/L to 350 mg/L were applied to maize seeds continuously for 7 days under in vitro condition. The experimental results showed that Its in high concentrations (250 and 350 mg/L) harm seed germination and seedling growth of maize, following the order: [Bmim]BF4 > [Omim]Cl > [Bmim]Cl. ILs treatment caused a reduction in seed germination, weight of root and shoot, leaf area, relative water content, photosynthetic pigments production. The antioxidant activity of maize seedling was decreased when the concentration of ILs was increased, thus in ILs stressed condition, the free radical could not be effectively scavenged. However, an increase in the phenolic content was observed when ILs concentration increased from 50 mgt to 350 mg/L It has been observed that increasing alkyl chain length attached to imidazolium cation of ILs from butyl to octyl harm the performance of maize seedling. Moreover, IL having BF4 anion is more toxic than ILs having Cl anion on maize growth seedling. (C) 2020 Elsevier B.V. All rights reserved.

关键词： 蓝墨云班课   信息化教学   教学设计  

摘要： 基于网络教学平台蓝墨云班课的翻转课堂作为一种全新的信息化教学模式已被普遍采用。本文以食品微生物学教学为例,以翻转课堂教学模式为基础,将蓝墨云班课移动网络教学平台应用于实践教学中,加强师生互动交流,提高教学质量和学习效率,为“互联网+教育”背景下,深化职业教育教学改革提供一定的借鉴与参考。

关键词： 食品工程原理   教学改革   自主学习   综合能力培养  

摘要： “食品工程原理”是一门重要基础专业课,做好其理论教学是该课程其他教学环节顺利进行的前提。在新工科背景人才培养的背景下,以培养学生自主学习能力为出发点,从课前、课上、课后3个方面阐述了“食品工程原理”理论教学改革的具体措施,实现“食品工程原理”理论教学效果提高,促进学生独立解决问题和团结协作等综合能力的培养。