关键词:
metabolism
expressing
Lycium
摘要:
Carotenoids are important natural pigments and have medical and health functions for *** cleavage dioxygenase 4(CCD4)and ethylene responsive factor(ERF)participate in carotenoid metabolism,but their roles in Lycium have not been ***,we annotated LbCCDs from the Lycium reference genome and found that LbCCD4.1 expression was significantly correlated with the carotenoid metabolites during Lycium five fruit developmental ***-expression of LbCCD4.1 in NQ’s leaves resulted in a series of significantly lower contents of carotenoid metabolites,includingβ-carotene andβ-***,LbERF5.1,a transcription factor belonging to the ERF family that was located in the nucleus,was *** reductions in the carotenoids,especially lutein,violaxanthin and their derivatives,were observed in over-expressing ERF5.1 transgenic NQ’s ***-expression or virus-induced gene silencing of LbERF5.1 in NQ’s leaves induced a consistent up-or down-expression,respectively,of ***,yeast one-hybrid and dual-luciferase reporter assays showed that ERF5.1 interacted with the promoter of CCD4.1 to increase its expression,and LbERF5.1 could bind to any one of the three predicted binding sites in the promoter of LbCCD4.1.A transcriptome analysis of LbERF5.1 and LbCCD4.1 over-expressed lines showed similar global transcript expression,and geranylgeranyl diphosphate synthase,phytoene synthase,lycopeneδ-cyclase cytochrome,cytochrome P450-type monooxygenase 97A,cytochrome P450-type monooxygenase 97C,and zeaxanthin epoxidase in the carotenoid biosynthesis pathway were differentially *** summary,we uncovered a novel molecular mechanism of carotenoid accumulation that involved an interaction between ERF5.1 and CCD4.1,which may be used to enhance carotenoid in Lycium.