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关键词： 市政桥梁工程   伸缩缝施工   质量控制  

摘要： 保证质量是市政桥梁建设的灵魂,而伸缩缝施工技术在市政桥梁建设中占有重要地位。通过在城市桥梁端部和桥台中心、两端设置伸缩缝,有效地调节车辆荷载和结构材料物理性能的差异。城市桥梁的质量是伸缩缝的主要目标。但在施工过程中还存在一些问题,由于平坦、伸缩缝不能通过验收,最终造成公路、桥梁交通不畅。在施工过程中,应运用现代施工管理控制理论和思想,完善和加强以往的施工质量控制措施,确保公路桥梁车辆安全稳定运行,并使各种建筑材料和施工程序在公路桥梁工程中的应用效果得到明显提高。伸缩缝施工时,施工人员应严格执行有关规章制度,把握其施工要点,选择相应的质量控制措施,以保证车辆行驶的舒适性,防止行车安全问题。本文通过对伸缩缝施工技术质量控制策略的分析,希望能给市政桥梁工程建设方提供一些借鉴和帮助,有效地解决当前伸缩缝施工中存在的问题。

摘要： Current challenges with the in vitro-in vivo extrapolation (IVIVE) of hepatic uptake clearance involving organic anion-transporting polypeptide (OATP) 161/163 hinder drug design strategies. Here we evaluated the effect of 100% human plasma on the uptake clearance using transfected human embryonic kidney (HEK) 293 cells and primary human hepatocytes and assessed IVIVE. Apparent unbound uptake clearance (PSinf,u) increased significantly (P < 0.05) in the presence of plasma (vs. buffer incubations) for about 50% of compounds in both OATP1B1-transfected and wild-type HEK cells. Thus, plasma showed a minimal effect on the uptake ratios. With cultured human hepatocytes, plasma significantly (P < 0.05) increased PSinf,u for 11 of 19 OATP16 substrates (median change of 2.1-fold). Cell accumulation in HEK cells and hepatocytes was also increased for tolbutamide, which is not an OATP substrate. Plasmato-buffer ratio of PSinf,u obtained in hepatocytes showed a good correlation with unbound fraction in plasma, and the relationship was best described by a "facilitated-dissociation" model. IVIVE was evaluated for the 19 OATP16 substrates using hepatocyte data in the presence of buffer and plasma. PSinf,u from buffer incubations markedly underpredicted hepatic intrinsic clearance (calculated via well stirred and parallel tube models) with an estimated bias of 0.10-0.13. Predictions improved when using PSinf,u from plasma incubations;however, considerable systemic underprediction was still apparent (0.19-0.26 bias). Plasma data with a global scaling factor of 3.8-5.3 showed good prediction accuracy (95% predictions within 3-fold;average fold error = 1.7, bias = 1). In summary, this study offers insight into the effect of plasma on the uptake clearance and its scope in improving IVIVE. SIGNIFICANCE STATEMENT Our study using diverse anionic compounds shows that human plasma facilitates organic anion-transporting polypeptide 16-mediated as well as passive uptake clearance, particularl

关键词： 城镇建筑物   遥感影像   建筑分类   震害风险  

摘要： 震害损失主要是由建筑物损毁造成的,对城镇建筑物进行有效分类可以做好震害风险防范,通过遥感影像信息提取的方法对建筑物进行分类能提高工作效率。采用多分割图层及多尺度分割技术,利用特征库阈值分类与样本最邻近分类相结合的方法对遥感影像建筑物进行信息提取及分类。分类结果精度评价表明该方法优于利用单一分割图层样本最近邻分类结果,可以用于城镇建筑物分类。根据建筑物分类结果对震害风险进行了划分。

关键词： altitudinal difference   Fargesia spathacea complex   genetic divergence   SSR   vegetative trait  

摘要： The taxonomy of woody bamboo presents many difficulties due to its long blooming interval and complex morphological *** the current taxonomy reflects genuine species divergence within woody bamboo is an intriguing *** Fargesia spathacea *** comprises 15 closely related species with a sympatric distribution in *** classification has long been controversial because only a handful of vegetative traits are available,providing a good opportunity to explore the evolutionary relationships and genetic differentiation in woody ***,we present a study involving 750 individuals from 39 representative populations in the *** complex using 14 simple sequence repeat *** found varying degrees of genetic diversity across populations of the *** complex (He =0.07-0.81) and largely negative F-values at the population level,implying an excess of heterozygotes in the *** analyses revealed that all populations were divided into two major groups (clusters A and B),with the majority of the 15 species representing distinct genetic *** on population genetic analysis along with morphological evidence,we confirmed the identity of three species (*** ***,***,and *** Gamble) and suggested the invalidation of four other species (*** ***,*** ***,*** *** *** (Gamble) ***,and *** (Mitford) Keng *** ***).The delimitation of the remaining eight species has yet to be *** analysis of ecological factors and spatial autocorrelation suggested that altitudinal differences might account for the distinct genetic divergence between the two major groups.

关键词： 低维护   装配式   屋顶绿化景观  

摘要： 在设计屋顶绿化景观时，低维护装配式是一种重要方式，这种设计方式简单且高效。在设计过程中，需体现出以人为本的思想理念，也需保证美观性与经济性。实际设计中，布局应主要运用地毯草坪，将乔木和灌木作为辅助，并且与不同场合实际需求和使用特征之间结合在一起，使不同功能需求获得充分满足。

关键词： 桥梁   伸缩缝   防水控制   技术措施  

摘要： 目前,我国的经济在迅猛发展,社会在不断进步,公路桥梁扩建连接中的混凝土裂缝明显。材料的特性和冷热扩展功能在市政桥梁建设中具有十分重要的意义。伸缩接头的防水特性可以极大地影响桥梁的功能。安装操作人员应合理为市政桥梁安装伸缩接缝,对城市桥梁扩建接缝进行全防水控制。因此,笔者将继续致力于市政桥梁扩建接缝的防水控制措施,希望能给同行带来一定的参考价值。

关键词： Single-cell RNA sequencing   Embryonic mouse brain   E15.5   Primary cortical cells   Cryopreservation  

摘要： Background: The application of single-cell RNA sequencing (scRNASeq) represents a unique approach to identify hundreds to millions of cells in mammalian cortical multilayers at different stages of embryogenesis. ScRNASeq technology applied to neurological studies requires the use of fresh starting materials because standard cryopreservation methods do not guarantee high viability of cortical primary cells derived from dissected brain areas. New method: Here we set up and validate an innovative strategy to perform scRNASeq studies in cryopreserved primary cortical cells isolated from E15.5 mouse embryo. In order to freeze cortical primary cells, we have employed Neurostore, a medium able to guarantee high viability and cell composition of embryonic cortex after thawing. Comparison with existing methods: We showed for the first time the possibility to run scRNASeq experiments on primary cortical cells in an off-line set-up, ensuring cellular integrity and diversity. Results: By trypan blue assay and flow cytometry analysis, we found that Neurostore-cryopreserved cortical cells showed approximately 95 % of viability. Satisfactory RNA recovery and cDNA libraries were achieved. Transcriptome sequencing of 35,763 cryoconserved single cells yielded a robust data-set, identifying 25 cell clusters in three biological samples. Prevalence of peculiar neural populations before and after the cryopreservationresuscitation procedure was verified by marker gene expression and immunofluorescence analysis. Conclusions: Our findings support the evidence that frozen primary cortical cells can be successfully employed in scRNASeq experiments allowing an unprecedented flexibility in experimental procedures, such as sample preparation and subsequent processing steps performed in different locations.

关键词： 轻质隔墙   建筑工程   装配式建筑  

摘要： 为有效解决建筑行业中工程进展速度慢、施工效率差等问题,本文将以轻质隔墙技术在装配式建筑工程中的应用展开研究,系统性地分析了轻质隔墙技术于建筑施工中诸如墙板安装、阴阳角等环节中存在的问题,并同时提出为预防墙体出现裂缝的解决对策,以期为建筑工程的相关人员提供参考.

关键词： 建筑集团公司   安全风险   分级动态管控  

摘要： 近年来,国务院安委办要求各地区、各有关部门和单位构建安全风险分级管控和隐患排查治理双重预防机制,各行业主管部门分别印发了相关指南和办法,但要求并不统一。建筑集团公司管理的施工项目达数百甚至数千个,且涉及到公路水运、市政、房建、水利水电、铁路等多个行业领域,本文就建筑集团公司安全风险分级动态管理进行探讨,以期为相关工作起到参考作用。

关键词： 物联网   智慧多功能灯杆   智慧节点   功能分区   运维平台  

摘要： 针对物联网时代智慧多功能灯杆的特有社会优势,总结分析当前智慧多功能灯杆的行业应用情况,充分了解了智慧多功能灯杆在未来5G物联网大数据时代中可承载的多种商业模式,并对智慧多功能灯杆的功能设计及整体解决方案进行了探讨,结合综合运维平台的功能设想提出了一些发展方向的想法。通过智慧多功能灯杆产品的优势和逐步成熟的多行业多维度商业模式的发展趋势,相信智慧多功能灯杆在未来的智慧城市、智慧园区以及无人驾驶等领域将会承担不可替代的角色。