关键词：
Citotoxic
Anti-inflammatory
Methanolic extract
Jatropha curcas L.
Astrocyte
Microglia
NF-KAPPA-B
REACTIVE GLIOSIS
NATURAL-PRODUCTS
MICROGLIA
ACTIVATION
ASTROCYTES
INFLAMMATION
AUTOPHAGY
DISEASE
INJURY
摘要：
Ethnopharmacological relevance: Jatropha curcas L. (Euphorbiaceae), a medicinal plant known in Brazil as "Pinhao Manso", is highly adaptable, being cultivated in different tropical and subtropical regions of the world. Antimicrobial, antioxidant and antiinflammatory activities have been attributed to different parts of the plant. In the central nervous sytem (CNS), neuroinflammation is mediated by glial cells, mainly by astrocytes and microglia, a process that plays an important role in neurodegenerative diseases and other CNS disorders. In this study, we investigated the anti-inflammatory activity of the methanolic extract obtained from the leaves of J. curcas L. (MEJc) in primary cultures of glial cells submited to inflammatory stimulus. Materials and methods: Primary cultures of glial cells obtained from the cerebral cortex of neonate Wistar rats were treated with MEJc (0.1-50,000 mu g mL(-1)) and its fractions (F(n)Jc) (0.1 mu g mL(-1)) with or without lipopolysaccharide of Escherichia coli (LPS) (1 mu g mL(-1)). Cell viability was determined with MTT test. Modifications in glial cell morphology were investigated by means of phase contrast microscopy and May-Grunwald staining. The reactivity of astrocytes and microglia were investigated with immunocytochemistry for GFAP, Iba1 and transcription factor NF-kB, as well as with Greiss reaction to determine the nitric oxide (NO) production. Results: MEJc at 0.1-1000 mu g mL(-1) was non-toxic to glial cells and the DE50 was 10.794 mu g mL(-1). The treatment with LPS induced the activation of astrocytes and microglia marked by morphological modifications and changes in the expression of GFAP and Iba1, as well as the increase in NF-kB expression and NO production. Treatment with MEJc inhibited the morphological modifications, changes in GFAP and Iba1 expression, and the increase in NF-kB and NO production induced by LPS. Conclusion: This study demonstrates that the MEJc and its fractions modulate inflammatory response of
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