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关键词： RIBOSOMAL-PROTEINS   CD19   THERAPY   PATHWAY   GENE  

摘要： Chimeric antigen receptor T cell (CAR-T) therapy has revolu-tionized the clinical treatment of hematological malignancies due to the prominent anti-tumor effects. B cell maturation antigen (BCMA) CAR-T cells have demonstrated promising effects in patients with relapsed/refractory multiple myeloma. However, the dynamics of CAR-T cell proliferation and cyto-toxicity in clinical patients remains unexplored. Here, we longi-tudinally profiled the transcriptomes of 55,488 T cells including CAR-T products, CAR-T cells, and endogenous T cells at the peak and remission phases in a plasma cell leuke-mia (PCL) patient treated with BCMA CAR-T cells by single -cell transcriptomic analysis. Our results showed distinct CAR-T and endogenous T cell subsets indicating stage-specific expression in proliferation, cytotoxicity, and intercellular signaling pathways. Furthermore, we found that CAR-T cells at peak phase gradually convert to a highly cytotoxic state from a highly proliferative state along a development trajec-tory. Moreover, re-analysis of a single cell study from CD8+ CD19 CAR-T confirmed our findings. These commonalities suggest conserved mechanisms for CAR-T treatment across he-matological malignancies. Taken together, our current study provides insight into CAR-T cell dynamics during CAR-T ther-apy and proves that both BCMA CAR-T and CD19 CAR-T have similar transcriptional characteristics, especially at the CAR-T peak phase.Y

关键词： binocular vision   chandelier cells   ocular dominance   development   callosal   visual cortex  

摘要： In mammals with binocular vision, integration of the left and right visual scene relies on information in the center visual field, which are relayed from each retina in parallel and merge in the primary visual cortex (V1) through the convergence of ipsi- and contralateral geniculocortical inputs as well as transcallosal projections between two visual cortices. The developmental assembly of this binocular circuit, especially the transcallosal pathway, remains incompletely understood. Using genetic methods in mice, we found that several days before eye-opening, retinal and callosal activities drive massive apoptosis of GABAergic chandelier cells (ChCs) in the binocular region of V1. Blockade of ChC elimination resulted in a contralateral eye-dominated V1 and deficient binocular vision. As pre-vision retinal activities convey the left-right organization of the visual field, their regulation of ChC density through the transcallosal pathway may prime a nascent binocular territory for subsequent experience-driven tuning during the post-vision critical period.

关键词： 装配式隔板   大跨度内隔墙   施工工艺  

摘要： 采用灰渣板、改性石膏板、复合轻质隔墙板等作为非承重内隔墙,通常采用传统施工工艺就可以满足设计使用要求,但大跨度内隔墙则存在墙面易开裂、安全性一般等问题。基于此,文章以具体工程为例,介绍了一种可以在大跨度内隔墙中应用的装配式隔墙板施工工艺,较好地解决了这些问题,具有一定的推广价值。

关键词： 建筑设计   变形缝   伸缩缝  

摘要： 变形缝在建筑中发挥协调变形,避免建筑结构被破坏的作用,可提高建筑稳定性,保障建筑施工安全。基于此,论文结合变形缝的类型,分析建筑设计中变形缝的作用,分析建筑设计中变形缝的构造要求,总结变形缝设计要点,为设计人员提供理论与实践帮助。

关键词： 道路   桥梁   工程伸缩缝   施工  

摘要： 随着我国经济的快速发展,道路和桥梁建设项目的数量在增加,项目建设的范围也在逐步扩大,伸缩缝施工是路桥技术建设的重要组成部分。它对提高工程建设的整体质量起着关键作用,将直接影响道路和桥梁使用的安全性和美观性。由于伸缩缝建造需要诸多环节,这给质量控制带来了很大的困难。因此,在伸缩缝施工中,有必要根据实际工程在施工中采取有效的技术质量措施,以提高路桥伸缩缝的施工质量,促进工程的顺利开展。

关键词： 节能环保材料   屋顶绿化技术   建筑节能设计  

摘要： 随着我国建筑行业的快速发展,社会对房屋建筑节能方面的要求有所提高。当前阶段,我国各种科学技术都得到了快速发展。在建筑行业中,除了需要对原有的节能技术进行推广之外,还可以结合一些功能优异的新型节能环保材料,从而进一步推动房屋建筑行业的快速发展。就目前形势来看,已经出现了许多屋顶绿化技术、节能环保材料等,将其有效运用到建筑节能设计工作中,能够产生积极效益。由此可见,研究节能环保材料与屋顶绿化技术在建筑节能设计中的应用具有积极的社会意义,希望本篇文章的发展能够对相关工作人员产生一定启示,更好地推动我国建筑业的快速发展。