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关键词： replicative senescence   aging   breast cancer   mammary epithelial cell   proteomics   data integration   CELLULAR SENESCENCE   SECRETORY PHENOTYPE   CANCER   EXPRESSION   DIFFERENTIATION   GROWTH  

摘要： Senescence is a permanent cell cycle arrest that occurs in response to cellular stress and promotes age-related disease. Because senescence differs greatly depending on cell type and senescence inducer, continued progress in the characterization of senescent cells is needed. Here, we analyzed primary human mammary epithelial cells (HMECs), a model system for aging and cancer, using mass spectrometry-based proteomics. By integrating data from replicative senescence, immortalization by telomerase reactivation, and quiescence, we identified a robust proteomic signature of HMEC senescence consisting of 34 upregulated and 10 downregulated proteins. This approach identified known senescence biomarkers including beta-galactosidase (GLB1) as well as novel senescence biomarkers including catechol O-methyltransferase (COMT), synaptic vesicle membrane protein VAT-1 homolog (VAT1), and plastin-1/3 (PLS1/PLS3). Gene ontology enrichment analysis demonstrated that senescent HMECs upregulated lysosomal proteins and downregulated RNA metabolic processes. In addition, a classification model based on our proteomic signature successfully discriminated proliferating and senescent HMECs at the transcriptional level. Finally, we found that the HMEC senescence signature was positively and negatively correlated with proteomic alterations in HMEC aging and breast cancer, respectively. Taken together, our results demonstrate the power of proteomics to identify cell type-specific signatures of senescence and advance the understanding of senescence in HMECs.

关键词： 装配式   复合轻质隔墙   接缝  

摘要： 文中分析了复合轻质墙板的优点,并从多方面阐述了预制装配式复合内隔墙施工技术。

摘要： Purpose/Objective(s) Prediction of extranodal extension (ENE) status of the involved lymph nodes has been shown to be an important factor in the prognosis and treatment of patients with Oropharyngeal Squamous Cell Carcinomas (OPSCC). Official diagnosis of ENE occurs pathologically at the time of surgery, and typically necessitates aggressive adjuvant treatment with radiation and concurrent chemotherapy. Due to higher toxicity with trimodality therapy, definitive chemoradiation can be a preferred treatment option up front instead of surgery if there is high clinical suspicion of ENE. This study investigates the potential value of radiomic features extracted from pre-treatment primary gross tumor volume (GTV) and clinical target volume (CTV) on diagnostic contrast enhanced Computed Tomography (CE-CT) images for prediction of ENE in patients with OPSCC. Materials/Methods For this study we identified 26 patients with pathologically confirmed OPSCC who went on to receive surgery and neck nodal dissection. Out of this group 13 were p16 positive, 13 were p16 negative, 7 were pathologic ENE positive, and 19 pathologic ENE negative. We contoured the primary tumor GTV on their pre-surgery CE-CT and added a 1 cm to create the CTV for radiomic analysis. For each patient, two sets of IBSI (Image Biomarker Standardization Initiative) validated radiomic features (N = 192 features from 11 different feature categories) were extracted from primary GTV and CTV on the diagnostic CE-CT images. Levene and Kolmogorov-Smirnov's (KS, P < 0.05) tests with confidence level of 95% along with absolute biserial correlation (|BSC|) with a threshold of > 0.2 were used to statistically reveal significant associations between ENE status and radiomics features. The Belsley collinearity diagnostics test was applied on the discriminant radiomic features for removing highly correlated features. Eight different Generalized-Linear Models (GLMs) were trained and tested using each individual feature along w

摘要： Purpose/Objective(s) Past studies have shown mixed results of postoperative radiation (PORT) for pN2 NSCLC patients and the recent LungART trial has shown no disease free survival or overall survival (OS) benefits with PORT for pN2 NSCLC patients. High lymph node ratio (LNR = number of positive lymph nodes in the resected specimen/number of total lymph nodes resected) has been shown to correlate with worse outcomes. We hypothesize that PORT can improve survival outcomes in pN2 NSCLC patients with high LNR. Materials/Methods The National Cancer Database was queried for cases of NSCLC from 2004-2016. Patients without neoadjuvant treatment , with at least a lobectomy with resection of at least 10 lymph nodes, with pN2 disease, with histology of squamous cell carcinoma, adenocarcinoma, adenosquamous carcinoma and large cell carcinoma were included. Exclusion criteria were neoadjuvant therapy, lack of pathological confirmation or lack of information about chemotherapy and metastatic disease . For patients with PORT, RT was directed to thorax with dose≥30Gy. Log-rank test and Cox proportional hazards models were used to compare survival adjusted for age, sex, CCI, pT, grade, chemotherapy, LNR, and PORT. Predictors for overall survival (OS) were identified. Results Among 10272 patients, 6266 (61%) did not have PORT, and 4006 (39%) had PORT. Median follow up was over 5 years. PORT was given to 51.3% and 37.7% patients with non-R0 and R0 resection, respectively ( P < 0.001). PORT was given to 48.4% and 9.91% of patients with and without adjuvant chemotherapy , respectively ( P < 0.001). The average LNR for patients with and without PORT were 0.33 and 0.29 ( P < 0.001), respectively. In multivariate analysis (MVA) of patients with R0 resection, predictors of OS were listed in Table 1. PORT was not associated with better OS (HR, 1.04, P  = 0.63) for patients with LNR of 1-15%. However, PORT was associated with better survival in patients with higher LNRs (HR, 0.90, P  = 0.23 f

关键词： 公路   桥梁伸缩缝   维修更换   养护  

摘要： 伸缩缝是公路桥梁主要构成部分,鉴于伸缩缝的特殊功效,容易致使桥梁运行形成问题,应对公路桥梁伸缩缝的维护更换和保养,有益于提升更换保养水准,进而保障公路桥梁安全行车。公路养护流程中,务必要搞好桥梁伸缩缝的维护保养,增强检修,适度开展更换工作,进而保障桥梁伸缩缝正常的效果的发挥。本文公路桥梁伸缩缝维修更换技术及其养护的措施、注意事项开展解析研究。

关键词： 西域都护府   乌拉泊古城   轮台   访古   功能分区  

摘要： 新疆历史上有两个轮台。一个是汉轮台,即西域都护府所在地乌垒城,现在巴州轮台县境内。另一个是唐轮台,即现在的乌拉泊古城,地处乌鲁木齐市南郊。周日的上午,艳阳高照,秋色初显,我们登临唐轮台故城。夯土筑成的城墙历历在目,虽然断壁残垣,但依然气势磅礴,风骨凛凛。城垣基本呈正方形,边长各里许。大城之内又划分为三个小城,作为不同的功能分区。

关键词： 隔断墙   电梯   轻质材料   房车   楼梯   阳台   别墅   自动排列  

摘要： 隔断墙德国公司研发的隔断墙会自动排列成墙,把大房间分隔成一个个小房间。每块活动墙是由复合轻质材料制成,上下都嵌在轨道中,可以自由移动形成隔墙。每一块墙就好像列兵一样,排成一队向前走,只要几分钟,一道墙就平地而起。带电梯的房车房车如何住出别墅的感觉?新型房车Life Home V90 Villa Edition,颠覆了传统的房车结构,你只要点一下按钮,车顶就会升起,变成一个二层小楼,并且,还有一个小阳台,方便晒太阳看星星。车内没有楼梯,采用一个单人电梯取代,方便在狭小的空间中升降。

关键词： 市政道路施工   伸缩缝施工技术   技术应用  

摘要： 本文的研究目的是为了明确伸缩缝施工技术在市政道路施工中的重要性,并分析了具体的应用。现阶段,在经济发展的要求下,我国交通道路的压力日益增多,问题逐渐突出。因此,为了解决这些问题,我国开展了相应的市政道路建设工程,并将伸缩缝施工技术应用其中。该技术可以降低材料对温度的敏感程度,调整性能差异,还能够减少车辆荷载对道路的破坏,因此,这项技术得到了广泛应用。但即便如此,该技术也存在一定的问题需要解决。

关键词： 建筑符号学   传统建筑   宁夏地区   地域文化性  

摘要： 随着地域性和本土化建筑的兴起,将传统的建筑符号运用到现代建筑中的现象越来越广泛。以宁夏地区传统建筑为例,从建筑符号的基本概述、宁夏传统建筑特征、宁夏传统建筑文化与符号论以及反思传统建筑符号对现代建筑的影响等四方面进行探析,以期使中国传统建筑符号能够通过更加有效的手段传承下去,运用到今后更多的现代建筑当中。