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关键词： Computer-assisted diagnosis system   Bone scan   Renal cell carcinoma   Bone metastases   Bone turnover marker   SCAN INDEX   CANCER-PATIENTS   KIDNEY CANCER   SYSTEM   BIOMARKERS   SURVIVAL  

摘要： Background Computer-assisted diagnosis (CAD) systems for bone scans have been introduced as clinical quality assurance tools, but few studies have reported on its utility for renal cell carcinoma (RCC) patients. The aim of this study was to assess the diagnostic validity of the CAD system for bone scans and to construct a novel diagnostic system for bone metastases in RCC patients. Methods We evaluated bone scan images of 300 RCC patients. Artificial neural network (ANN) values, which represent the probability of abnormality, were calculated by BONENAVI, the CAD software for bone scans. By analyzing ANN values, we assessed the diagnostic validity of BONENAVI. Next, we selected 108 patients who underwent measurements of bone turnover markers and assessed the combined diagnostic validity of BONENAVI and bone turnover markers. Results Forty-three out of 300 RCC patients had bone metastases. The AUC of ANN values was 0.764 and the optimum sensitivity and specificity were 83.7 and 62.7%. By logistic analysis of 108 cases, we found that ICTP, a bone resorption marker, could be a diagnostic marker. The AUC of ICTP was 0.776 and the optimum sensitivity and specificity were 57.1 and 86.8%. Subsequently, we developed a novel diagnostic model based on ANN values and ICTP. Using this model, the AUC was 0.849 and the optimum sensitivity and specificity were 76.2 and 80.7%. Conclusion By combining the high sensitivity provided by BONENAVI and the high specificity provided by ICTP, we constructed a novel, high-accuracy diagnostic model for bone metastases in RCC patients.

关键词： Esophageal squamous cell carcinoma   Multidetector computed tomography   Tumor staging   POSITRON-EMISSION-TOMOGRAPHY   ENDOSCOPIC ULTRASOUND   COMPUTED-TOMOGRAPHY   CANCER   ULTRASONOGRAPHY   ACCURACY   PET/CT  

摘要： Background CT is the most commonly used method to stage esophageal cancer (EC). However, the reported CT T-staging criteria for EC are controversial. Purpose To determine and validate the optimal esophageal wall thickness (EWT) threshold on CT to distinguish lesions with different T stages in esophageal squamous cell carcinoma (ESCC) patients. Methods One thousand, one hundred-two consecutive patients with histopathologically confirmed ESCC between July 2014 and April 2020 were retrospectively reviewed. All patients underwent a preoperative CT examination and surgical treatment. The maximal EWT of the lesions on CT was measured. Patients were divided into pT1, pT2, pT3 and pT4 subgroups according to the pathologic stage. We employed the support vector machine, where linear kernels were leveraged to determine the optimal threshold to classify samples with different T stages. 90% of samples from each subgroup were randomly selected as the training set, while the remainder comprised the testing set. Results The mean EWTs of the pT1, pT2, pT3 and pT4 subgroups were 4.9 +/- 2.6 mm, 8.1 +/- 2.3 mm, 12.4 +/- 3.6 mm, and 18.6 +/- 4.4 mm, respectively. Differences in the EWT between the four subgroups or between adjacent subgroups were significant (p < 0.001), and esophageal wall became thicker with increasing pT stage. We utilized MATLAB 2020a to implement the SVM model and ran the code 10 times. The accuracy of the model was 60.29 +/- 2.33%. The thresholds between samples from pT1/pT2, pT2/pT3 and pT3/pT4 lesions were 5.5 +/- 0.3 mm, 10.8 +/- 0.8 mm and 15.9 +/- 0.5 mm, respectively. Conclusions Possibility of predicting T stage of ESCC by EWT on CT scans was limited to 60% by model examination with large sample size.

关键词： Lymphocytes   NK cells   NKT-like cells   Primary Sjogren's syndrome   INNATE   IMMUNITY  

摘要： Objective To reveal the characteristics and potential role of natural killer T-like cells (NKT-like cells) in the pathogenesis of primary Sjogren's syndrome (pSS). Methods Forty-six patients with pSS and 30 healthy subjects were enrolled in the study. The frequencies and cell count of NKT-like cells as well as other lymphocyte subsets were analyzed by flow cytometry. The clinical and laboratory indicators of pSS patients were also collected. Then, the correlation between NKT-like cells and pSS patient manifestations was analyzed by Spearman's rank test. In addition, NKT-like cells before and after therapy were also compared. Results Both the number and the frequencies of NKT-like cells were significantly decreased in pSS patients. The counts of NKT-like cells were positively correlated with CD4(+) T cells (r = 0.464, P = 0.001), CD8(+) T cells (r = 0.363, P = 0.013), NK cells (r = 0.488, P = 0.001), and IgM levels (r = 0.443, P = 0.002), while negatively correlated with the disease duration (r = - 0.33, P = 0.027). Moreover, after effective therapy, NKT-like cells were recovered both in the cell counts and frequencies. Conclusion In pSS, NKT-like cells were fundamentally decreased, potentially contributing to the disease pathogenesis. Modulating the status of NKT-like cells might provide a novel strategy for treating the disease.

关键词： AC16   HL-1   Cardiomyocyte   Stem cell   Primary cell culture   MIGRATION INHIBITORY FACTOR   PLURIPOTENT STEM-CELLS   INSULIN-RESISTANCE   HL-1 CELLS   IN-VITRO   H9C2   DIFFERENTIATION   EXPRESSION   HYPERTROPHY   METABOLISM  

摘要： Background: Cardiac cell lines and primary cells are widely used in cardiovascular research. Despite increasing number of publications using these models, comparative characterization of these cell lines has not been performed, therefore, their limitations are undetermined. We aimed to compare cardiac cell lines to primary cardiomyocytes and to mature cardiac tissues in a systematic manner. Methods and results: Cardiac cell lines (H9C2, AC16, HL-1) were differentiated with widely used protocols. Left ventricular tissue, neonatal primary cardiomyocytes, and human induced pluripotent stem cell-derived cardiomyocytes served as reference tissue or cells. RNA expression of cardiac markers (e.g. Tnnt2, Ryr2) was markedly lower in cell lines compared to references. Differentiation induced increase in cardiac-and decrease in embryonic markers however, the overall transcriptomic profile and annotation to relevant biological processes showed consistently less pronounced cardiac phenotype in all cell lines in comparison to the corresponding references. Immunocytochemistry confirmed low expressions of structural protein sarcomeric alpha-actinin, troponin I and caveolin-3 in cell lines. Susceptibility of cell lines to sI/R injury in terms of viability as well as mitochondrial polarization differed from the primary cells irrespective of their degree of differentiation. Conclusion: Expression patterns of cardiomyocyte markers and whole transcriptomic profile, as well as response to sI/R, and to hypertrophic stimuli indicate low-to-moderate similarity of cell lines to primary cells/cardiac

关键词： HSTCs   ASCs   A-Reg   Hepatokines   Adipokines   Metabolic syndrome   Crosstalk   OBESITY   DISEASE  

摘要： Herein, we would like to introduce a novel concept for the prevention and treatment of metabolic syndrome, which is based on molecular relationship between liver and adipose tissue. Particularly, we believe, that unravelling the molecular crosstalk between hepatokines and adipokines will allow to better understand the pathophysiology of metabolic diseases and allow to develop novel, effective therapeutic solutions against obesity and metabolic syndrome.

摘要： 一 你吃过维C泡腾片吗? 一粒橘粉色的药片,丢进装满白开水的杯子,时间仿佛固化,变成气泡,扑腾翻滚,像火山爆发过的痕迹. 泡腾片小姐就是这样的人,一点就着,随时随地会以自己的情绪为中心,影响方圆十里. 她第一次见到镇定先生真人是在厨房. 尽管在南方长大,但当蟑螂出现在水槽里时,泡腾片小姐还是忍不住尖叫起来.她这个人天不怕地不怕,唯独蟑螂是她的克星.这一吼,把其余两间屋子的室友都吓了出来.