关键词:
ELECTROSPRAY MASS SPECTROMETRY
PROTEIN PHOSPHORYLATION
PHOSPHOGLYCERATE MUTASE
SACCHAROMYCES-CEREVISIAE
PROTEINS
SEQUENCE
摘要:
Electrospray mass spectrometry has been used to study the formation and hydrolysis of the phopshorylated forms of two phosphoglycerate mutases, The half-life of the enzyme from Saccharomyces cerevisiae was 35 min at 20 degrees C in 10 mM ammonium bicarbonate, pH 8.0, Addition of 1 mM 2-phosphogly-collate reduced this value by at least 100-fold, The phosphorylated form of the enzyme from Schizosaccharomyces pombe was much less stable with a half-life of less than 1 min, The results are discussed in terms of the kinetic properties of the enzymes, Mass spectrometry would appear to be a powerful method to study the formation and breakdown of phosphorylated proteins, processes which are of widespread significance in regulatory mechanisms.