关键词:
OLIGONUCLEOTIDES
DIGESTION
PCR
摘要:
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and ionpair-reverse-phase high-performance liquid chromatography (IP-RP HPLC) techniques were combined to determine the sequence identity of short single-stranded deoxyoligonucleotides. This methodology is demonstrated using a commercially available multiplex set of eight primer pairs. The primer pairs were separated and collected by IP-RP HPLC. Partial sequence information for IP-RP HPLC fractions was obtained from analyzing exonuclease digestion products by MALDI-TOF MS. IP-RP HPLC, MALDI-TOF MS, exonuclease digests, and a simple computational algorithm provide an integrated strategy for determining the sequence of short nucleic acid oligomers. (C) 2002 Elsevier Science (USA).