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关键词： 尿糖   微量白蛋白   糖尿病肾病   早期   诊断  

摘要： 目的探讨尿糖(uGlu)与微量白蛋白(mAlb)检测指标在早期诊断糖尿病肾病中的意义。方法将该院100例糖尿病肾病患者作为观察组,采用干化学试纸检测尿糖,采用免疫胶体金技术检测微量白蛋白在晨尿中的水平,同时与对照组100例健康体检者的相关体检指标进行对比。结果糖尿病肾病患者中尿微量白蛋白含量呈阳性的有56例,尿糖呈阳性的有81例,明显高于健康体检者,且与发展为糖尿病肾脏的患者数相符。结论尿糖和微量白蛋白检验同时呈阳性是早期确诊糖尿病肾病存在的有效手段。

关键词： 临床检验基础   高职生   可持续发展能力  

摘要： 可持续发展的本质是人的全面、持续发展，是新时代高等教育培养可持续发展人才的根本指导方针，作为培养就业大军的高等职业院校肩负着培养学生可持续发展能力的重大责任。专业知识和技能作为高职生可持续发展能力的重要方面，一直被用人单位和学校所看重，对这种能力的培养主要通过教学活动来实现。临床检验基础作为高职高专医学检验技术专业的核心课程，

关键词： 交通肇事   物证   检验技术   作用  

摘要： 随着经济的发展交通技术的进步,随之而来的也遇到了一些难题,比如交通事故的发生。然而据资料分析,交警部门在处理交通肇事案件中,在运用痕迹检验技术勘验此类案件时,更多的时候是根据特殊的痕迹来检验肇事案件,即通过对整体分离痕迹和轮胎印痕来认定肇事车辆,很少关注其他痕迹在事件中的作用,更是很少注意到侦查方向的特殊作用。该文通过一系列具体的交通肇事案件,通过在交通肇事案件痕迹检验技术的运用以及现场侦查方向的运用,从而以最有效的方式侦破交通肇事案件。

关键词： Imidazolium based ionic liquid   Apparent molar volume   Apparent molar isentropic compression   Density   Speed of sound   AQUEOUS-SOLUTIONS   THERMODYNAMIC PROPERTIES   MOLECULAR-DYNAMICS   COMPRESSIBILITY   BEHAVIOR   TETRAFLUOROBORATE   ANION  

摘要： Densities and speeds of sound for aqueous solutions of ionic liquids having 1-butyl-3-methylimidazolium as cation and chloride, bromide, iodide, acetate, tetrafluoroborate, and trifluoromethanesulfonate as anions were accurately measured at various concentrations and temperatures. The data were used in evaluating thermodynamic properties as apparent molar volumes and apparent molar isentropic compressions. Infinite dilution values of these properties were determined using appropriate extrapolation procedures utilizing Debye-Huckel limiting law for electrolyte solutions. Apparent molar isobaric expansions at infinite dilutions were also evaluated from the temperature dependence of apparent molar volumes. The results were interpreted in terms of ionic liquid-water interactions. (C) 2012 Elsevier Ltd. All rights reserved.

关键词： metagenomics   taxonomic binning   next-generation sequencing   PHYLOGENETIC CLASSIFICATION   COMMUNITY STRUCTURE   FUNCTIONAL-ANALYSIS   MAXIMUM-LIKELIHOOD   DNA-SEQUENCES   WEB SERVER   GENOMES   PLACEMENT   DIVERSITY   DATABASE  

摘要： Metagenome research uses random shotgun sequencing of microbial community DNA to study the genetic sequences of its members without cultivation. This development has been strongly supported by improvements in sequencing technologies, which have rendered sequencing cheaper than before. As a consequence, downstream computational analysis of metagenome sequence samples is now faced with large amounts of complex data. One of the essential steps in metagenome analysis is reconstruction of draft genomes for populations of a community or of draft 'pan-genomes' for higher level clades. 'Taxonomic binning' corresponds to the process of assigning a taxonomic identifier to sequence fragments, based on information such as sequence similarity, sequence composition or read coverage. This is used for draft genome reconstruction, if sequencing coverage is insufficient for reconstruction based on assembly information alone. Subsequent functional and metabolic annotation of draft genomes allows a genome-level analysis of novel uncultured microbial species and even inference of their cultivation requirements.

关键词： TUMOR CLASSIFICATION   FEATURE-SELECTION   SKELETAL-MUSCLE   NEMALINE MYOPATHY   MARKER GENES   R-PACKAGE   CANCER   ALGORITHMS   PREDICTION   RANKING  

摘要： Background: A feature selection method in microarray gene expression data should be independent of platform, disease and dataset size. Our hypothesis is that among the statistically significant ranked genes in a gene list, there should be clusters of genes that share similar biological functions related to the investigated disease. Thus, instead of keeping N top ranked genes, it would be more appropriate to define and keep a number of gene cluster exemplars. Results: We propose a hybrid FS method (mAP-KL), which combines multiple hypothesis testing and affinity propagation (AP)-clustering algorithm along with the Krzanowski & Lai cluster quality index, to select a small yet informative subset of genes. We applied mAP-KL on real microarray data, as well as on simulated data, and compared its performance against 13 other feature selection approaches. Across a variety of diseases and number of samples, mAP-KL presents competitive classification results, particularly in neuromuscular diseases, where its overall AUC score was 0.91. Furthermore, mAP-KL generates concise yet biologically relevant and informative N-gene expression signatures, which can serve as a valuable tool for diagnostic and prognostic purposes, as well as a source of potential disease biomarkers in a broad range of diseases. Conclusions: mAP-KL is a data-driven and classifier-independent hybrid feature selection method, which applies to any disease classification problem based on microarray data, regardless of the available samples. Combining multiple hypothesis testing and AP leads to subsets of genes, which classify unknown samples from both, small and large patient cohorts with high accuracy.

关键词： Fire debris   Factor analysis   Bayesian decision theory   Pattern classification   Chemometrics   IGNITABLE LIQUIDS   GASOLINE SAMPLES   COVARIANCE  

摘要： A Bayesian soft classification method combined with target factor analysis (TFA) is described and tested for the analysis of fire debris data. The method relies on analysis of the average mass spectrum across the chromatographic profile (i.e., the total ion spectrum, TIS) from multiple samples taken from a single fire scene. A library of TIS from reference ignitable liquids with assigned ASTM classification is used as the target factors in TFA. The class-conditional distributions of correlations between the target and predicted factors for each ASTM class are represented by kernel functions and analyzed by Bayesian decision theory. The soft classification approach assists in assessing the probability that ignitable liquid residue from a specific ASTM E1618 class, is present in a set of samples from a single fire scene, even in the presence of unspecified background contributions from pyrolysis products. The method is demonstrated with sample data sets and then tested on laboratory-scale burn data and large-scale field test burns. The overall performance achieved in laboratory and field test of the method is approximately 80% correct classification of fire debris samples. (C) 2012 Elsevier Ireland Ltd. All rights reserved.

关键词： Macrophage   Lipopolysaccharide   Sub-clinical inflammation   Arginine metabolism   Redox regulation   Biomarker   NITRIC-OXIDE SYNTHASE   GAMMA-GLUTAMYL-TRANSFERASE   MONOCYTE CHEMOATTRACTANT PROTEIN-1   FACTOR-KAPPA-B   ARGININE METABOLISM   ARGININOSUCCINATE SYNTHETASE   ENDOTHELIAL DYSFUNCTION   PULMONARY-HYPERTENSION   ACTIVATOR PROTEIN-1   RISK-FACTORS  

摘要： Background: Shifts in intracellular arginine (Arg) and sulfur amino acid (SAA) redox metabolism modulate macrophage activation, polarization and phenotype. Despite their importance in inflammation and redox regulatory pathways, comprehensive analysis of these metabolic networks was not previously possible with existing analytical methods. Methods: The Arg/thiol redox LC-MS/MS metabolomics assay permits simultaneous assessment of amino acids and derivative products generated from Arg and SAA metabolism. Using this assay, LPS-induced changes in macrophage amino acid metabolism were monitored to identify pathway shifts during activation and their linkage to cellular redox regulation. Results: Metabolite concentrations most significantly changed after treatment of a macrophage-like cell line (RAW) with LPS for 24 hrs were citrulline (Cit) (48-fold increase), ornithine (Orn) (8.5-fold increase), arginine (Arg) (66% decrease), and aspartic acid (Asp) (73% decrease). The ratio Cit + Orn/Arg + Asp (CO/AA) was more sensitive to LPS stimulation than other amino acid ratios commonly used to measure LPS-dependent inflammation (e.g., SAM/SAH, GSH/GSSG) and total media NOx. The CO/AA ratio was also the first ratio to change significantly after LPS treatment (4 hrs). Changes in the overall metabolomic profile over time indicated that metabolic pathways shifted from Arg catabolism to thiol oxidation. Conclusions: Simultaneous quantification of Arg and SAA metabolic pathway shifts following LPS challenge of macrophage indicate that, in this system, the Arg- Citrulline/NO cycle and arginase pathways are the amino acid metabolic pathways most sensitive to LPS- challenge. The cellular (Cit + Orn)/(Arg + Asp) ratio, which summarizes this pathway, was more responsive to lower concentrations of LPS and responded earlier than other metabolic biomarkers of macrophage activation including GSH redox. It is suggested that the CO/ AA ratio is a redoxindependent early biomarker of macrophage act

关键词： 检验   临床   诊疗  

摘要： 本文简明扼要地分析了在现代医学飞速发展的今天,在循证医学在临床医学中被得到广泛应用的时代,检验与临床密切配合的重要意义,并在此基础上详细介绍了检验与临床密切配合的方法及其诊疗作用。

关键词： 书面形式   劳动合同   口头约定   部门主管   公司   工资   张某  

摘要： 问：张某于2011年6月与一家公司签订了一份为期两年的劳动合同，约定其担任技术指导，月工资3000元，4个月后，公司经与公司协商，决定由其担任部门主管，月工资上调到4500元。但双方就此变更只是口头约定，而未采用书面形式。此后张某一直在新岗位上班，公司也一直按新标准发放工资。