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关键词： SP-NOV.   DIGESTIVE-TRACT   GEN.-NOV.   FECES   IDENTIFICATION   GUT   LACTOBACILLUS   DIVERSITY   PROPOSAL   STRAINS  

摘要： Occurrence of bifidobacteria, known as health-promoting probiotic microorganisms, in the digestive tract of wild pigs (Sus scrofa) has not been examined yet. One hundred forty-nine fructose-6-phosphate phosphoketolase positive bacterial strains were isolated from colonic content of twenty-two individuals of wild pigs originated from four localities in the Czechia. Based on PCR-DGGE technique targeting the variable V3 region of the 16S rRNA genes, strains were initially differentiated into four groups represented by: (i) probably a new Bifidobacterium species (89 strains), (ii) B. boum/B. thermophilum/B. thermacidophilum subsp. porcinum/B. thermacidophilum subsp. thermacidophilum (sub)species (49 strains), (iii) Pseudoscardovia suis (7 strains), and (iv) B. pseudolongum subsp. globosum/B. pseudolongum subsp. pseudolongum (4 strains), respectively. Given the fact that DGGE technique did not allow to differentiate the representatives of thermophilic bifidobacteria and B. pseudolongum subspecies, strains were further classified by the 16S rRNA and thrS gene sequences. Primers targeting the variable regions of the latter gene were designed to be applicable in identification and phylogeny of Bifidobacteriaceae family. The 16S rRNA-derived phylogenetic study classified members of the first group into five subgroups in a separated cluster of thermophilic bifidobacteria. Comparable results were obtained by the thrS-derived phylogenetic analysis. Remarkably, variability among thrS sequences was higher compared with 16S rRNA gene sequences. Overall, molecular genetic techniques application allowed to identify a new Bifidobacterium phylotype which is predominant in the digestive tract of examined wild pigs.

关键词： Ensemble Kalman filter   Data assimilation   Hydrologic ensemble prediction   Interaction   Streamfiow   Uncertainty   POSSIBILISTIC OPTIMIZATION FRAMEWORK   WATER-RESOURCES MANAGEMENT   MONTE-CARLO   MODEL PARAMETERS   STREAMFLOW DATA   UNCERTAINTY   STATE   PREDICTION   REDUCTION   INFERENCE  

摘要： The ensemble Kalman filter (EnKF) is recognized as a powerful data assimilation technique that generates an ensemble of model variables through stochastic perturbations of forcing data and observations. However, relatively little guidance exists with regard to the proper specification of the magnitude of the perturbation and the ensemble size, posing a significant challenge in optimally implementing the EnKF. This paper presents a robust data assimilation system (RDAS), in which a multi-factorial design of the EnKF experiments is first proposed for hydrologic ensemble predictions. A multi-way analysis of variance is then used to examine potential interactions among factors affecting the EnKF experiments, achieving optimality of the RDAS with maximized performance of hydrologic predictions. The RDAS is applied to the Xiangxi River watershed which is the most representative watershed in China's Three Gorges Reservoir region to demonstrate its validity and applicability. Results reveal that the pairwise interaction between perturbed precipitation and streamflow observations has the most significant impact on the performance of the EnKF system, and their interactions vary dynamically across different settings of the ensemble size and the evapotranspiration perturbation. In addition, the interactions among experimental factors vary greatly in magnitude and direction depending on different statistical metrics for model evaluation including the Nash-Sutcliffe efficiency and the Box Cox transformed root-mean-square error. It is thus necessary to test various evaluation metrics in order to enhance the robustness of hydrologic prediction systems. (C) 2017 Elsevier B.V. All rights reserved.

关键词： Protein-protein interaction   Negative samples   Feature selection   Ensemble classifier   Protein-protein non-interactions   IDENTIFICATION   DNA   NETWORKS   DATABASE   MODES  

摘要： Computational methods are employed in bioinformatics to predict protein-protein interactions (PPIs). PPIs and protein-protein non-interactions (PPNIs) display different levels of development, and the number of PPIs is considerably greater than that of PPNIs. This significant difference in the number of PPIs and PPNIs increases the cost of constructing a balanced dataset. PPIs can be classified as either physical or genetic. However, ready-made PPNI databases were proven only to have no physical interactions and were not proven to have no genetic interactions. Hence, ready-made PPNI databases contain false negative non-interactions. In this study, two PPNI datasets were artificially generated from a PPI database. In contrast to various traditional PPI feature extraction methods based on sequential information, two types of novel feature extraction methods were proposed. One is based on secondary structure information, and the other is based on the physicochemical properties of proteins. The experimental results of the RandomPairs dataset validate the efficiency and effectiveness of the proposed prediction model. These results reveal the potential of constructing a PPI negative dataset to reduce false negatives. Related datasets, tools, and source codes are accessible at http://***/PPIPre/***. (C) 2017 Elsevier B.V. All rights reserved.

关键词： 产品质量检验   质量技术监督   作用  

摘要： 人们生活在不断改善的同时,更加看重物质质量需求。为此,技术监督工作的开展,一定要认识到产品质量检验在中间所起的重要作用。本文通过对质量技术监督中产品质量检验的作用的研究,为质量技术监督工作的开展提供指导,促使其更好的开展,以期为完善质量技术监督中产品质量检验理论内容提供借鉴意义。

关键词： 肝硬化   生化验项目   诊断  

摘要： 目的探讨肝硬化疾病采用生化检验项目诊断的作用。方法选择肝硬化患者72例,均为本院2015年6月~2016年6月收治,设为观察组,同期选取本院健康体检者60例,设为对照组,分别抽取两组受试者清晨、空腹状态下静脉血5 m L,对血清样本开展生化检验,对各检验项目检测结果进行比较。结果观察组肝硬化患者γ-谷氨酰转移酶、总胆固醇、碱性磷酸酶、直接胆红素、天冬氨酸氨基转移酶、总胆汁酸指标相较正常范围,均居较高水平,而胆碱酯酶、总蛋白、白蛋白与球蛋白比值、白蛋白指标相较正常范围,均居较低水平。两组上述各项生化指标进行对比,差异具有统计学意义(P<0.05)。结论针对临床收治的肝硬化患者,在诊断时,通过检测生化检验项目,可为临床对肝功能状态的掌握提供参考依据,以便以此为参考,对预防和治疗措施进行制定,延缓肝硬化发展进程,对维护患者健康、最大程度改善疾病预后意义重大,具有非常重要的开展价值。

关键词： Biological sample characterization   Image analysis   Atomic force microscopy   Endothelial cells   LUNG EPITHELIAL-CELLS   CARBON NANOTUBES   ENGINEERED NANOMATERIALS   QUANTITATIVE-ANALYSIS   ULTRASOUND IMAGES   SURFACE   AFM   MAMMOGRAMS   ROUGHNESS   PARTICLES  

摘要： The morphological changes induced by engineered nanomaterials (ENMs) in cellular samples are a key clue to evaluate the impact of these materials on human health. The investigation of the complexity of the interaction among nanoparticles and cellular molecules requires cutting-edge instrumentation and dedicated procedures. To this regard, atomic force microscopy (AFM) is a leading imaging technique that has the peculiarities of high resolution and direct relationship with 3D cellular morphology. Expert human operators, however, are still required to manage most of the AFM-based analysis, thus introducing subjective bias and allowing a limited number of trials. The modality of interaction and the resulting topographic differences are often not a priori known. Also, the presence of artefacts in AFM images may affect the derived conclusions. In this work, we propose a robust and flexible strategy to analyse AFM topography images with single click-select actions by the human operator. The proposed system allows for not only morphological studies and quantification of the changes occurring in cellular samples in the presence of nanomaterials, but also for the investigation of diversified experiments in more flexible application domains. As a proof of concepts, samples of human EA. hy926 endothelial cells exposed to carbon nanotubes are used to demonstrate the effectiveness of the proposed solution. The system is also tested against various AFM-artefact and noise scenarios and the robustness of its discrimination capability is verified. (C) 2017 Elsevier B.V. All rights reserved.

关键词： HbA1c   FPG   妊娠期糖尿病(GDM)   OGCT检验  

摘要： 选择62例患有GDM而于2016年01月-2017年03月进入本院的患者,为甲组,同期选取健康孕妇70例作乙组,均施以Hb A1c检验、FPG检验以及OGCT检验。结果:甲组HbA_(1c)值(79.00±9.26)%,乙组(46.99±6.22)%,(P<0.05);同时甲组FPG值及其OGCT值也比乙组高,(P<0.05)。且HbA_(1c)技术阳性检出率比FPG技术以及OGCT技术高,(P<0.05)。结论:检验Hb A1c,可实现对患者疾病症状的准确判断。

关键词： 抗生素合理使用   感染患者   微生物   检验技术   住院患者   抗生素滥用   感染发生率   院内感染  

摘要： 院内感染是患者住院期间发生的感染，近年来，随住院患者增多、抗生素滥用，院内感染发生率呈不断上升趋势，其可引起各个系统并发症，且表现复杂多变，成为威胁住院患者生命安全、影响预后的重要因素。抗生素是一种能干扰菌类微生物发育功能的化学物质，

关键词： 技术优势   实践证明   技术服务   进口货物   检验检疫技术   莫兰   消杀   中心设置   不合格   风险信息  

摘要： 近年来,厦门出入境检验检疫局立足发挥自身技术优势,不断致力探索参与社区治理,通过加大口岸文明共建力度,特别是在公共食品安全、社区防疫减灾等方面,提供了富有特色的技术服务。实践证明,检验检疫部门主动融入社区共治体系,有利于巩固文明共建成果。