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关键词： 雨课堂   教学模式   应用探究  

摘要： 随着时代的发展,教学手段呈现多样化趋势,许多新型平台和媒体手段为广大教师的教学过程提供了便利的条件,“雨课堂”以其自身优势受到诸多教师的青睐。药学生物化学是一门知识体系非常庞大、覆盖面非常广、知识更新频繁的学科。作为实践类课程利用雨课堂平台既丰富了知识体系,又改变了授课方式,改善了教学效果,突破了传统教学手段的局限,提升了学习积极性,从而培养了学生扎实的学科基础,以及严谨、细致、求实、创新的科学作风。

关键词： 生物化学   生物化学实验   教学改革   卓越医生   教学模式   多元化考核  

摘要： 2013年,遵义医科大学启动卓越医生教育培养计划,推动多学科课程教学模式的改革,其中生物化学实验通过改革课程设置,合理分配教学学时;课程细化,完善课程教学;优化实验教学,灵活采用网络教学、讨论式教学等多种教学模式;考核多元化,综合评定学生学习情况,关注个别差异,以期达到综合评定学生的标准,进而激发学生主观能动性,认知自我,积极参与教学过程,使得实验内容体现出基础性、综合性、设计性、创新性,呈现出层层递进、不断提高的模式,培养了学生的自主学习能力、创新意识、团队合作精神,并启蒙与引导学生的科学创新和设计能力,收获了良好的教学效果。

关键词： 云班课平台   线上线下混合式教学   教学效果   测试成绩   小组任务   自主学习积极性   线上线下混合教学   《动物生物化学》  

摘要： 为了研究基于云班课平台的线上线下混合式教学模式,进行了《动物生物化学》线上线下混合教学实践,对获得的云班课数据和教学效果进行了分析,结果表明:64.71%的学生云班课资源学习低于60%;云班课测试分数46.16%学生在60分以下,作业/小组任务分数33.33%学生测试成绩在60分以下,教师需进一步研究增加学生自主学习积极性的方法,达到更优的教学效果。

关键词： Salt-tolerant Actinomycetes   Submerged cultivation   Thermostable protease   Thermodynamics   Circular dichroism spectroscopy   ORGANIC-SOLVENT   OPTIMIZATION   DETERGENT   ENZYME   FERMENTATION   ACTINOMYCETE   QUANTITIES   PARAMETERS   BACTERIUM   ACID  

摘要： The Nocardiopsis alba strain OM-5 showed maximum protease production in submerged culture. The OM-5 protease was purified by hydrophobic interaction chromatography. The purified protease of 68 kDa showed maximum activity (3312 +/- 1.64 U/mL) at 70 degrees C and was quite stable at 80 degrees C up to 4 M NaCl (w/v) at pH 9. The purified protease showed significant activity and stability in different cations, denaturing agents, metal ions, and osmolytes. The thermodynamic parameters including deactivation rate constant (K-d) and half lives (t(1/2)) at 50-80 degrees C were in the range of 2.50 x 10(-3) to 5.50 x 10(-3) and 277.25-111.25 min respectively at 0-4 M NaCl. The structural stability of the OM-5 protease under various harsh conditions was elucidated by circular dichroism (CD) spectroscopy followed by K2D3 analysis revealed that the native structure of OM-5 protease was stable even in sodium dodecyl sulfate and Tween 20 indicated by increased a-helices content assisted with decreased beta-sheets content. (C) 2020 Elsevier B.V. All rights reserved.

关键词： 生物化学   线上线下   混合教学  

摘要： 生物化学是研究生命现象及其本质的一门学科,其在医药领域的地位举足轻重。通过探索线上线下混合教学的模式,全面梳理课程内容,开拓课程方向,以提高学生上课的兴趣,拓宽学生的视野,解决传统线下教学的问题。因此希望通过本文的研究能够为线上线下教学模式提供一定的帮助。

关键词： Fumonisin   Carboxylesterase   Biological detoxification   CLASSIFICATION   MYCOTOXINS   DISEASE  

摘要： Fumonisins have posed hazardous threat to human and animal health worldwide. Enzymatic degradation is a desirable detoxification approach but is severely hindered by serious shortage of detoxification enzymes. After mining enzymes by bioinformatics analysis, a novel carboxylesterase FumDSB from Sphingomonadales bacterium was expressed in Escherichia coli, and confirmed to catalyze fumonisin B1 to produce hydrolyzed fumonisin B1 by liquid chromatography mass spectrometry for the first time. FumDSB showed high sequence novelty, sharing only similar to 34% sequence identity with three reported fumonisin detoxification carboxylesterases. Besides, FumDSB displayed its high degrading activity at 30-40 degrees C within a broad pH range from 6.0 to 9.0, which is perfectly suitable to be used in animal physiological condition. It also exhibited excellent pH stability and moderate thermostability. This study provides a FB1 detoxification carboxylesterase which could be further used as a potential food and feed additive. (C) 2020 Published by Elsevier B.V.

关键词： 生物物理学   学术期刊   系统生物学   神经科学   细胞生物学   栏目设置   学科范围   基因组学  

摘要： (2020年12月修订)1征稿范围与栏目设置1.1征稿范围本刊为国内外公开发行的全国性学术期刊,月刊,每月20日发行.征稿的学科范围是生物化学、分子生物学、生物物理学、细胞生物学、神经科学、免疫学,以及基因组学、蛋白质组学、系统生物学等相关学科领域.

关键词： OTR   5-HTR2C   Heteroreceptor complexes   GPCR crosstalk   Hypoactivity   5-HT2C RECEPTOR   INTRANASAL OXYTOCIN   SOCIAL COGNITION   DEVELOPMENTAL-HYPERSEROTONEMIA   ADMINISTERED OXYTOCIN   VASOPRESSIN V1A   AGONIST   LIGAND   TRAFFICKING   ACTIVATION  

摘要： The complexity of oxytocin-mediated functions is strongly associated with its modulatory effects on other neurotransmission systems, including the serotonin (5-hydroxytryptamine, 5-HT) system. Signalling between oxytocin (OT) and 5-HT has been demonstrated during neurodevelopment and in the regulation of specific emotion-based behaviours. It is suggested that crosstalk between neurotransmitters is driven by interaction between their specific receptors, particularly the oxytocin receptor (OTR) and the 5-hydroxytryptamine 2C receptor (5-HTR2C), but evidence for this and the downstream signalling consequences that follow are lacking. Considering the overlapping central expression profiles and shared involvement of OTR and 5-HTR2C in certain endocrine functions and behaviours, including eating behaviour, social interaction and locomotor activity, we investigated the existence of functionally active OTR/5-HTR2C heterocomplexes. Here, we demonstrate evidence for a potential physical interaction between OTR and 5-HTR2C in vitro in a cellular expression system using flow cytometry-based FRET (fcFRET). We could recapitulate this finding under endogenous expression levels of both receptors via in silico analysis of single cell transcriptomic data and ex vivo proximity ligation assay (PLA). Next, we show that co-expression of the OTR/5-HTR2C pair resulted in a significant depletion of OTR-mediated G alpha q-signalling and significant changes in receptor trafficking. Of note, attenuation of OTR-mediated downstream signalling was restored following pharmacological blockade of the 5-HTR2C. Finally, we demonstrated a functional relevance of this novel heterocomplex, in vivo, as 5-HTR2C antagonism increased OT-mediated hypoactivity in mice. Overall, we provide compelling evidence for the formation of functionally active OTR/5-HTR2C heterocomplexes, adding another level of complexity to OTR and 5-HTR2C signalling functionality. This article is part of the special issue on Neuropepti

关键词： 生物化学   线上教学   课程建设   自主学习  

摘要： 在疫情防控的特殊背景下,教师基于"超星学习通"平台构建了生物化学在线课程。教师通过利用优质丰富的教学资源,注重引入思政教育,构建合理有效的评价体系,保证教学活动顺利开展。在线学习实践和调查反馈显示,学生对在线课程大多持认可态度,并希望今后能继续开展线上教学方式。教师充分认识到线上教学由应急化转向常态化,需将传统教学的优势和线上学习的优势结合起来,充分发挥学生的主观能动性,达到最优的教育教学过程和最佳的学习效果。

关键词： 生物化学实验教学   学习兴趣   科学素养  

摘要： 生物化学作为医学教育的一门基础学科,其课程主干的实验部分是培养医学学生科学素养的关键环节。基于当前生物化学实验教学模式、教学内容、教学方式和教学考核的现状,本文探讨如何激发学生学习的兴趣,从而有助于培养和提高医学学生的科学素养。